Various lysates were subjected to SDS PAGE followed by western blot with 10541-1-AP (Calmodulin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10541-1-AP (Calmodulin antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10541-1-AP (Calmodulin antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IP result of anti-Calmodulin (IP:10541-1-AP, 2ug; Detection:10541-1-AP 1:600) with Ramos cells lysate 1120 ug.
1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Calmodulin (10541-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).