WB result of Calpain 1 antibody (10538-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Calpain 1 transfected HeLa cells.

Various lysates were subjected to SDS PAGE followed by western blot with 10538-1-AP (Calpain 1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

Immunofluorescent analysis of Hela cells, using CAPN1 antibody 10538-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

IP result of anti-Calpain 1 (IP:10538-1-AP, 4ug; Detection:10538-1-AP 1:1000) with HeLa cells lysate 1200ug.

Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 10538-1-AP (Calpain 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 10538-1-AP (Calpain 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

1X10^6 HeLa cells were stained with 0.2ug Calpain 1 antibody (10538-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

Various lysates were subjected to SDS PAGE followed by western blot with 11472-1-AP (Calpain 2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

MDA-MB-231 (WT and CAPN2 KO) lysates prepared with RIPA buffer, 50 μg protein loaded. 11472-1-AP incubated at 1:500 at 4°C overnight in 5% BSA in TBST. Ponceau stained transfers shown on right. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

WB result of Calpain 2 antibody (11472-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Calpain 2 transfected HeLa cells.

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11472-1-AP (Calpain 2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11472-1-AP (Calpain 2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Various lysates were subjected to SDS PAGE followed by western blot with 11472-1-AP (Calpain 2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

MDA-MB-231 lysates prepared and IP of CAPN2 performed using 1.0 μg of 11472-1-AP coupled to protein A- Sepharose beads. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

MDA-MB-231 WT cells (yellow outline) and SYT1 KO cells (red outline) labelled with a green or a far red fluorescence dye, respectively. Cells fixed with 4% PFA and stained with 11472-1-AP at 1:300, plus DAPI. Bars = 10 μm. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

Immunofluorescent analysis of HepG2 cells, using CAPN2 antibody 11472-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

Various lysates were subjected to SDS PAGE followed by western blot with 11472-1-AP (Calpain 2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

human stomach tissue were subjected to SDS PAGE followed by western blot with 17556-1-AP (Calpain 9 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human stomach cancer using 17556-1-AP (Calpain 9 antibody) at dilution of 1:100 (under 10x lens).

Immunohistochemical analysis of paraffin-embedded human stomach cancer using 17556-1-AP (Calpain 9 antibody) at dilution of 1:100 (under 40x lens).

Immunofluorescent analysis of HepG2 cells, using CAPN9 antibody 17556-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

PC-3 cells were subjected to SDS PAGE followed by western blot with 13675-1-AP (Calpain 11 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13675-1-AP (Calpain 11 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of (-20℃ Ethanol) fixed PC-3 cells using 13675-1-AP (Calpain 11 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 10415-1-AP (Calpain 3 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 10415-1-AP (Calpain 3 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 10415-1-AP (Calpain 3 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

mouse skin tissue were subjected to SDS PAGE followed by western blot with 15803-1-AP (Calpain S2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

IP Result of anti-Calpain S2 (IP:15803-1-AP, 4ug; Detection:15803-1-AP 1:300) with mouse skin tissue lysate 2000ug.

1X10^6 HeLa cells were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human Calpain 1 (CL647-10538) (red), or 0.2 ug isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Various lysates were subjected to SDS PAGE followed by western blot with 12250-1-AP (Calpastatin antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12250-1-AP (Calpastatin antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12250-1-AP (Calpastatin antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

IP Result of anti-Calpastatin (IP:12250-1-AP, 4ug; Detection:12250-1-AP 1:1000) with K-562 cells lysate 4000ug.

Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Calpastatin antibody (12250-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human Calpastatin (12250-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).