Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CoraLite®488-conjugated TSG101 antibody (CL488-67381, Clone: 2B7G8 ) at dilution of 1:200.

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Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CoraLite®488-conjugated TSG101 antibody (CL488-67381, Clone: 2B7G8 ) at dilution of 1:200.

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1X10^6 THP-1 cells were stained with 0.2 ug CoraLite®488 Anti-Human TSG101 (CL488-67381, Clone: 2B7G8) (red), or 0.2 ug CoraLite®488 Mouse IgG1 Isotype Control (CL488-66360, Clone: T1F8D3F10) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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1x10^6 C57BL/c mouse splenocytes were intracellularly stained with 0.25 ug Anti-Mouse Foxp3 (3G3) (65089-1-Ig, Clone: 3G3) or 0.25 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21), FITC anti-Mouse IgG1 Antibody, and CoraLite® Plus 647 Anti-Mouse CD4 (GK1.5) (CL647-65104, Clone: GK1.5). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 65089-1-Ig (Foxp3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 65089-1-Ig (Foxp3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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1X10^6 BALB/c mouse splenocytes were surface stained with PE Anti-Mouse CD4 (GK1.5) and then fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. Cells were then intracellularly stained with 0.06 ug APC Anti-Mouse Foxp3 (APC-65089, Clone:3G3) or 0.06 ug APC Mouse IgG1 Isotype Control (MOPC-21). It is recommended that the antibody dosage should not exceed 0.06ug.

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Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite®594 Lamin B1 antibody (CL594-66095, Clone: 3C10G12 ) at dilution of 1:200.

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1X10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite®594 Anti-Human Lamin B1 (CL594-66095, Clone:3C10G12) (red), or 0.8 ug CoraLite®594 Mouse IgG1 Isotype Control (MOPC-21) (CL594-65124, Clone: MOPC-21) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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1X10^6 mouse splenocytes were surface stained with CoraLite® Plus 647-conjugated Anti-Mouse CD4 (GK1.5) (CL647-65104, Clone: GK1.5) and then fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Cells were then stained with CoraLite® Plus 488-conjugated mouse IgG1 isotype control or 5 ul CoraLite® Plus 488-conjugated Anti-Mouse Foxp3 (CL488-65089, Clone: 3G3).

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1X10^6 K-562 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human c-MYC (CL488-67447, Clone:3D9C12) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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1X10^6 K-562 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human c-MYC (CL488-67447, Clone:3D9C12) (red), or 0.8 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human DNAJB1 (CL488-13174) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Immunofluorescent analysis of (4% PFA) fixed human thyroid cancer tissue using CL488-60207 (Galectin-3 antibody) at dilution of 1:50.

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Immunofluorescent analysis of (4% PFA) fixed human thyroid cancer tissue using CL488-60207 (Galectin-3 antibody) at dilution of 1:50.

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1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human Galectin-3 (CL488-60207, Clone:1C1B2) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL488-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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