Various lysates were subjected to SDS PAGE followed by western blot with 17917-1-AP (EIF4B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using EIF4B antibody (17917-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human EIF4B (17917-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CoraLite® Plus 488 EIF4B antibody (CL488-17917) at dilution of 1:200.

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1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human EIF4B (CL488-17917) (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Non-treated PC-3 and Calyculin A treated PC-3 cells were subjected to SDS PAGE followed by western blot with 28779-1-AP (Phospho-EIF4B (Ser406) antibody) at dilution of 1:60000 incubated at 4℃ overnight. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

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Non-treated and Calyculin A treated cells were subjected to SDS PAGE followed by western blot with 80059-1-RR (Phospho-EIF4B (Ser406) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Non-treated and Calyculin A treated cells were subjected to SDS PAGE followed by western blot with 80059-1-RR (Phospho-EIF4B (Ser406) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80059-1-PBS in a different storage buffer formulation.

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Non-treated and Calyculin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 28781-1-AP (Phospho-EIF4B (Ser422) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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Non-treated and Calyculin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 82550-1-RR (Phospho-EIF4B (Ser422) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin antibody as loading control.

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Non-treated and Calyculin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 82550-1-RR (Phospho-EIF4B (Ser422) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin antibody as loading control. This data was developed using the same antibody clone with 82550-1-PBS in a different storage buffer formulation.

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