Various lysates were subjected to SDS PAGE followed by western blot with 11149-1-AP (EIF4E antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human breast cancer using 11149-1-AP (EIF4E antibody) at dilution of 1:100 (under 40x lens).

Immunofluorescent analysis of HepG2 cells, using EIF4E antibody 11149-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

1X10^6 HepG2 cells were stained with 0.2ug EIF4E antibody (11149-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

WB result of EIF4E antibody (11149-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-EIF4E transfected HEK-293 cells.

Various lysates were subjected to SDS PAGE followed by western blot with 11149-1-AP (EIF4E antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

Various lysates were subjected to SDS PAGE followed by western blot with 66655-1-Ig (EIF4E antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66655-1-Ig (EIF4E antibody) at dilution of 1:5000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 66655-1-Ig (EIF4E antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

WB result of EIF4E antibody (66655-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EIF4E transfected HEK-293 cells.

1X10^6 HepG2 cells were intracellularly stained with 0.2 ug Anti-Human EIF4E (66655-1-Ig, Clone:3C6B9) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Various lysates were subjected to SDS PAGE followed by western blot with 66655-1-Ig (EIF4E antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CL488-66655 (EIF4E antibody) at dilution of 1:100.

1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human EIF4E (CL488-66655, Clone:3C6B9) (red), or 0.4 ug Mouse IgG1 Isotype Control (CL488-66360, Clone: T1F8D3F10) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human EIF4E (CL488-11149) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

1X10^6 HeLa cells were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human EIF4E (CL647-66655, Clone:3C6B9) (red), or 0.2 ug APC-65128 ; APC Mouse IgG2b Isotype Control (APC-65128, Clone: MPC-11) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

1X10^6 HepG2 cells were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human EIF4E (CL647-11149) (red), or 0.2 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CL594-66655 (EIF4E antibody) at dilution of 1:100.

1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human EIF4E (CL594-66655, Clone:3C6B9) (red), or 0.4 ug Mouse IgG1 Isotype Control (CL594-66360, Clone: T1F8D3F10) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Various lysates were subjected to SDS PAGE followed by western blot with 66655-1-Ig (EIF4E antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66655-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66655-1-Ig (EIF4E antibody) at dilution of 1:5000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66655-1-PBS in a different storage buffer formulation.

Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 66655-1-Ig (EIF4E antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66655-1-PBS in a different storage buffer formulation.

WB result of EIF4E antibody (66655-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EIF4E transfected HEK-293 cells. This data was developed using the same antibody clone with 66655-1-PBS in a different storage buffer formulation.

1X10^6 HepG2 cells were intracellularly stained with 0.2 ug Anti-Human EIF4E (66655-1-Ig, Clone:3C6B9) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66655-1-PBS in a different storage buffer formulation.

Various lysates were subjected to SDS PAGE followed by western blot with 66655-1-Ig (EIF4E antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66655-1-PBS in a different storage buffer formulation.