CHO cells were subjected to SDS PAGE followed by western blot with 12572-1-AP (EPAC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 12572-1-AP (EPAC1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 12572-1-AP (EPAC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using 12572-1-AP (EPAC1 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Various lysates were subjected to SDS PAGE followed by western blot with 12572-1-AP (EPAC1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

RAPGEF3 fusion protein Ag28284 SDS-PAGE

RAPGEF3 fusion protein Ag29369 SDS-PAGE

mouse brain tissue were subjected to SDS PAGE followed by western blot with 19103-1-AP (EPAC2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

IP Result of anti-EPAC2 (IP:19103-1-AP, 4ug; Detection:19103-1-AP 1:600) with mouse brain tissue lysate 2560ug.

human brain tissue were subjected to SDS PAGE followed by western blot with 12556-1-AP (RAPGEF5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Various lysates were subjected to SDS PAGE followed by western blot with 67044-1-Ig (EPAC2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

Various lysates were subjected to SDS PAGE followed by western blot with 67044-1-Ig (EPAC2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67044-1-PBS in a different storage buffer formulation.

RAPGEF4 fusion protein Ag5186 SDS-PAGE