Various lysates were subjected to SDS PAGE followed by western blot with 21535-1-AP (FOXO4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 21535-1-AP (FOXO4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using KHC1656 (FOXO4 IHC Kit).

Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using KHC1656 (FOXO4 IHC Kit).

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using KHC1656 (FOXO4 IHC Kit).

Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using KHC1656 (FOXO4 IHC Kit).

Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using KHC1656 (FOXO4 IHC Kit).

Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using KHC1656 (FOXO4 IHC Kit).

Various lysates were subjected to SDS PAGE followed by western blot with 18592-1-AP (FOXO1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

IP result of anti-FOXO1 (IP:18592-1-AP, 4ug; Detection:18592-1-AP 1:2000) with HepG2 cells lysate 960 ug.

Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using FOXO1 antibody (18592-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18592-1-AP (FOXO1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human FOXO1 (18592-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

WB result of FOXO3A antibody (10849-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FOXO3A transfected HEK-293 cells.

Various lysates were subjected to SDS PAGE followed by western blot with 10849-1-AP (FOXO3A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human breast cancer using 10849-1-AP (FOXO3A antibody) at dilution of 1:50 (under 40x lens).

IP result of anti-FOXO3A (IP:10849-1-AP, 4ug; Detection:10849-1-AP 1:8000) with HEK-293 cells lysate 920 ug.

Various lysates were subjected to SDS PAGE followed by western blot with 66428-1-Ig (FOXO3A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

IP result of anti-FOXO3A (IP:66428-1-Ig, 4ug; Detection:66428-1-Ig 1:1000) with HEK-293 cells lysate 2200 ug.

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66428-1-Ig (FOXO3A antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of (4% PFA ) fixed HepG2 cells using 66428-1-Ig(FOXO3A antibody) at dilution of 1:50 and Alexa Fluor 594-conjugated AffiniPure Goat Anti-mouse IgG(H+L).

WB result of FOXO3A antibody (66428-1-Ig, 1:12,000) with si-Control and si-FOXO3A transfected HEK293 cells.

Various lysates were subjected to SDS PAGE followed by western blot with 66428-1-Ig (FOXO3A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

mouse brain tissue were subjected to SDS PAGE followed by western blot with 19122-1-AP (FOXO6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Various lysates were subjected to SDS PAGE followed by western blot with 19122-1-AP (FOXO6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

IP Result of anti-FOXO6 (IP:19122-1-AP, 3ug; Detection:19122-1-AP 1:300) with mouse brain tissue lysate 4000ug.

Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19122-1-AP (FOXO6 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19122-1-AP (FOXO6 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Various lysates were subjected to SDS PAGE followed by western blot with 66457-1-Ig (FOXO1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

WB result of FOXO1 antibody (66457-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FOXO1 transfected HEK-293 cells.

Non-treated PC-3 and Calyculin A treated PC-3 cells were subjected to SDS PAGE followed by western blot with 28755-1-AP (Phospho-FOXO3A (Ser315) antibody) at dilution of 1:1000 incubated at 4℃ overnight. The membrane was stripped and re-blotted with GAPDH antibody as loading control.