Various lysates were subjected to SDS PAGE followed by western blot with 18592-1-AP (FOXO1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

IP result of anti-FOXO1 (IP:18592-1-AP, 4ug; Detection:18592-1-AP 1:2000) with HepG2 cells lysate 960 ug.

Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using FOXO1 antibody (18592-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18592-1-AP (FOXO1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human FOXO1 (18592-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Various lysates were subjected to SDS PAGE followed by western blot with 66457-1-Ig (FOXO1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

WB result of FOXO1 antibody (66457-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FOXO1 transfected HEK-293 cells.

Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite®488 FOXO1 antibody (CL488-18592) at dilution of 1:200.

1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®488 Anti-Human FOXO1 (CL488-18592) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

1X10^6 HeLa cells were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human FOXO1 (CL647-18592) (red), or 0.2 ug CL647 rabbit Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Various lysates were subjected to SDS PAGE followed by western blot with 66457-1-Ig (FOXO1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66457-1-PBS in a different storage buffer formulation.

WB result of FOXO1 antibody (66457-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FOXO1 transfected HEK-293 cells. This data was developed using the same antibody clone with 66457-1-PBS in a different storage buffer formulation.

Various lysates were subjected to SDS PAGE followed by western blot with 82997-1-RR (FOXO1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

Cytometric bead array standard curve ofMP00024-2, FOXO1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 82997-2-PBS. Detection antibody: 82997-3-PBS. Standard: Ag13296. Range: 1.25-20 ng/mL.

Various lysates were subjected to SDS PAGE followed by western blot with 82997-1-RR (FOXO1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82997-1-PBS in a different storage buffer formulation.

Cytometric bead array standard curve ofMP00024-1, FOXO1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 82997-1-PBS. Detection antibody: 82997-3-PBS. Standard: Ag13296. Range: 0.625-20 ng/mL.