WB result of GSK3B antibody (22104-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GSK3B transfected A549 cells.
Various lysates were subjected to SDS PAGE followed by western blot with 22104-1-AP (GSK3B antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
IP Result of anti-GSK3B (IP:22104-1-AP, 4ug; Detection:22104-1-AP 1:1000) with mouse brain tissue lysate 4000ug.
Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using GSK3B antibody (22104-1-AP) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 22104-1-AP (GSK3B Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 22104-1-AP (GSK3B Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using GSK3B antibody (22104-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).