Various lysates were subjected to SDS PAGE followed by western blot with 10566-1-AP (GYS1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 10566-1-AP (GYS1 antibody) at dilution of 1:200 (under 10x lens).

Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 10566-1-AP (GYS1 antibody) at dilution of 1:200 (under 40x lens).

IP Result of anti-GYS1 (IP:10566-1-AP, 4ug; Detection:10566-1-AP 1:300) with mouse skeletal muscle tissue lysate 2200ug.

Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 10566-1-AP (GYS1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

1X10^6 HepG2 cells were stained with 0.2ug GYS1 antibody (10566-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

1X10^6 HepG2 cells were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human GYS1 (CL647-10566) (red), or 0.2 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Immunohistochemical analysis of paraffin-embedded human colon tissue slide using KHC0498 (GYS1 IHC Kit).

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using KHC0498 (GYS1 IHC Kit).

Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using KHC0498 (GYS1 IHC Kit).

Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using KHC0498 (GYS1 IHC Kit).

Immunohistochemical analysis of paraffin-embedded human liver tissue slide using KHC0498 (GYS1 IHC Kit).

Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using KHC0498 (GYS1 IHC Kit).

Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using KHC0498 (GYS1 IHC Kit).

Non-treated PC-3 cells, phosphatase inhibitor treated and λ phosphatase PC-3 cells were subjected to SDS PAGE followed by western blot with 28855-1-AP (Phospho-GYS1 (Ser641) antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

Immunofluorescent analysis of (4% PFA) fixed λ phosphatase treated PC-3 cells using Phospho-GYS1 (Ser641) antibody (28855-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

Various lysates were subjected to SDS PAGE followed by western blot with 22371-1-AP (GYS2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. Mouse heart/ kidney/ skeletal muscle are negative control.

IP result of anti-GYS2 (IP:22371-1-AP, 4ug; Detection:22371-1-AP 1:1000) with rat liver tissue lysate 4000 ug.

Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 22371-1-AP (GYS2 antibody) at dilution of 1:200 (under 10x lens).

Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 22371-1-AP (GYS2 antibody) at dilution of 1:200 (under 40x lens).