Various lysates were subjected to SDS PAGE followed by western blot with 10197-1-AP (HDAC1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
IP Result of anti-HDAC1 (IP:10197-1-AP, 3ug; Detection:10197-1-AP 1:500) with mouse testis tissue lysate 10000ug.
Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 10197-1-AP (HDAC1 antibody) at dilution of 1:50 (under 40x lens).
Immunofluorescent analysis of SH-SY5Y cells, using HDAC1 antibody 10197-1-AP at 1:50 dilution and FITC-labeled donkey anti-rabbit IgG (green).
1X10^6 HeLa cells were stained with 0.2ug HDAC1 antibody (10197-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
Western blot analysis of HDAC1 in various cell lines using Proteintech antibody 66085-1-Ig at dilution of 1:50000 incubated at room temperature for 1.5 hours.
Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66085-1-Ig (HDAC1 Antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunofluorescent analysis of (10% Formaldehyde ) fixed HeLa cells using 66085-1-Ig(HDAC1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).