Various lysates were subjected to SDS PAGE followed by western blot with 10197-1-AP (HDAC1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 10197-1-AP (HDAC1 antibody) at dilution of 1:50 (under 40x lens).

Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HDAC1 antibody (10197-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

IP result of anti-HDAC1 (IP:10197-1-AP, 4ug; Detection:10197-1-AP 1:6000) with HeLa cells lysate 1600 ug.

1X10^6 HeLa cells were stained with 0.2ug HDAC1 antibody (10197-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

Various lysates were subjected to SDS PAGE followed by western blot with 66085-1-Ig (HDAC1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66085-1-Ig (HDAC1 Antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of (4% PFA) fixed A431 cells using HDAC1 antibody (66085-1-Ig, Clone: 4C4G7 ) at dilution of 1:12000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

Immunofluorescent analysis of (4% PFA) fixed A431 cells using CoraLite®488 HDAC1 antibody (CL488-66085, Clone: 4C4G7 ) at dilution of 1:2000, CL594-Phalloidin (red).

1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®488 Anti-Human HDAC1 (CL488-66085, Clone:4C4G7) (red), or 0.4 ug Mouse IgG1 Isotype Control (CL488-66360, Clone: T1F8D3F10) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human HDAC1 (CL488-10197) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Immunofluorescent analysis of (4% PFA) fixed A431 cells using CoraLite®647 HDAC1 antibody (CL647-66085, Clone: 4C4G7 ) at dilution of 1:1000.

1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®647 Anti-Human HDAC1 (CL647-66085, Clone:4C4G7) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

1X10^6 HeLa cells were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human HDAC1 (CL647-10197) (red), or 0.2 ug Control Antibody (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Immunofluorescent analysis of (4% PFA) fixed A431 cells using CoraLite®555 HDAC1 antibody (CL555-66085, Clone: 4C4G7 ) at dilution of 1:8000.

Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CL594-66085 (HDAC1 antibody) at dilution of 1:100.

1X10^6 HeLa cells were intracellularly stained with 0.2 ug CoraLite®594 Anti-Human HDAC1 (CL594-66085, Clone:4C4G7) (red), or 0.2 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Immunofluorescent analysis of (4% PFA) fixed A431 cells using CoraLite®594 HDAC1 antibody (CL594-66085, Clone: 4C4G7 ) at dilution of 1:8000.

Immunofluorescent analysis of (4% PFA) fixed A431 cells using CoraLite®594 HDAC1 antibody (CL594-66085, Clone: 4C4G7 ) at dilution of 1:8000.