1X10^6 SK-BR-3 cells were intracellularly stained with 0.4 ug Anti-Human HER2/ErbB2 (60311-1-Ig, Clone:1B12A7) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (66360-3-Ig, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 60311-1-PBS in a different storage buffer formulation.
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60311-1-Ig (HER2/ErbB2 antibody) at dilution of 1:1600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60311-1-PBS in a different storage buffer formulation.
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60311-1-Ig (HER2/ErbB2 antibody) at dilution of 1:1600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60311-1-PBS in a different storage buffer formulation.
Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using 60311-1-Ig (HER2/ErbB2 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60311-1-PBS in a different storage buffer formulation.
Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using 60311-1-Ig (HER2/ErbB2 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60311-1-PBS in a different storage buffer formulation.