WB result of HNRNPC antibody (11760-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HNRNPC transfected HeLa cells.
Various lysates were subjected to SDS PAGE followed by western blot with 11760-1-AP (HNRNPC antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
IP result of anti-HNRNPC(IP:11760-1-AP, 4ug; Detection:11760-1-AP 1:10000) with HeLa cells lysate 1360 ug.
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 11760-1-AP (HNRNPC antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HNRNPC antibody (11760-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
1X10^6 HeLa cells were stained with 0.2ug HNRNPC antibody (11760-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.