Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using RNF31 antibody (83137-3-RR, Clone: 230446H1 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83137-3-PBS in a different storage buffer formulation.
1x10^6 PC-3 cells were intracellularly stained with 0.2 ug Rnf31 Recombinant Antibody (83137-3-RR, Clone:230446H1) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.2 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83137-3-PBS in a different storage buffer formulation.
Cytometric bead array standard curve of MP00285-1, RNF31 Recombinant Matched Antibody Pair - PBS only. Capture antibody: 83137-3-PBS. Detection antibody: 83137-1-PBS. Standard: Ag34505. Range: 1.25-20 ng/mL.
Cytometric bead array standard curve of MP00285-2, RNF31 Recombinant Matched Antibody Pair - PBS only. Capture antibody: 83137-3-PBS. Detection antibody: 83137-2-PBS. Standard: Ag34505. Range: 1.25-20 ng/mL.