Various lysates were subjected to SDS PAGE followed by western blot with 18284-1-AP (HSP27 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 18284-1-AP (HSP27 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Various lysates were subjected to SDS PAGE followed by western blot with 18284-1-AP (HSP27 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 18284-1-AP (HSP27 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

1X10^6 MCF-7 cells were intracellularly stained with 0.5 ug Anti-Human HSP27 (18284-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 18284-1-AP (HSP27 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

WB result of HSP27 antibody (18284-1-AP; 1:60000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HSP27 transfected HeLa cells.

Various lysates were subjected to SDS PAGE followed by western blot with 66767-1-Ig (HSPB1 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66767-1-Ig (HSPB1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

1X10^6 HepG2 cells were intracellularly stained with 0.5 ug Anti-Human HSP27 (66767-1-Ig, Clone:3E7A1) (red) labeled with FlexAble CoraLite® Plus 555 Antibody Labeling Kit for Mouse IgG1 (KFA022), or 0.5 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Antibody staining on tissue sections of the zebrafish retina at 5 days post fertilisation (dpf) for Heatshock protein 27 (Hsp27;magenta); (#66767-1-Ig) Dilution 1:200. Tissue fixed overnight in 4% PFA; DAPI, blue. Data generated by Natalia Jaroszynska in Professor Ryan MacDonald's lab, University College London, UK.

WB result of HSP27 antibody (66767-1-Ig; 1:60000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HSP27 transfected HeLa cells.

Non-treated HeLa and TNF alpha (HZ-1014) treated HeLa cells were subjected to SDS PAGE followed by western blot with 28900-1-AP (Phospho-HSP27 (Ser78) antibody) at dilution of 1:1000 incubated at 4℃ overnight. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CoraLite®488 HSP27 antibody (CL488-66767, Clone: 3E7A1 ) at dilution of 1:200.

1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug CoraLite®488 Anti-Human HSP27 (CL488-66767, Clone:3E7A1) (red), or 0.4 ug Mouse IgG1 Isotype Control (CL488-66360, Clone: T1F8D3F10) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

1X10^6 HepG2 cells were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human HSP27 (CL647-66767, Clone:3E7A1) (red), or 0.2 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using CoraLite®594 HSP27 antibody (CL594-66767, Clone: 3E7A1 ) at dilution of 1:200.

Various lysates were subjected to SDS PAGE followed by western blot with 66767-1-Ig (HSPB1 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66767-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66767-1-Ig (HSPB1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66767-1-PBS in a different storage buffer formulation.

1X10^6 HepG2 cells were intracellularly stained with 0.5 ug Anti-Human HSP27 (66767-1-Ig, Clone:3E7A1) (red) labeled with FlexAble CoraLite® Plus 555 Antibody Labeling Kit for Mouse IgG1 (KFA022), or 0.5 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66767-1-PBS in a different storage buffer formulation.

Antibody staining on tissue sections of the zebrafish retina at 5 days post fertilisation (dpf) for Heatshock protein 27 (Hsp27;magenta); (#66767-1-Ig) Dilution 1:200. Tissue fixed overnight in 4% PFA; DAPI, blue. Data generated by Natalia Jaroszynska in Professor Ryan MacDonald's lab, University College London, UK. This data was developed using the same antibody clone with 66767-1-PBS in a different storage buffer formulation.

WB result of HSP27 antibody (66767-1-Ig; 1:60000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HSP27 transfected HeLa cells. This data was developed using the same antibody clone with 66767-1-PBS in a different storage buffer formulation.

Non-treated HeLa cells, and anisomycin treated HeLa cells were subjected to SDS PAGE followed by western blot with 83332-3-RR (Phospho-HSP27 (Ser15) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin(66009-1-Ig) antibody as loading control. This data was developed using the same antibody clone with 83332-3-PBS in a different storage buffer formulation.