WB result of IGF2BP1 antibody (22803-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-IGF2BP1 transfected Jurkat cells.
IP result of anti-IGF2BP1 (IP:22803-1-AP, 4ug; Detection:22803-1-AP 1:1000) with HEK-293 cells lysate 1480ug.
Various lysates were subjected to SDS PAGE followed by western blot with 22803-1-AP (IGF2BP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
Immunofluorescent analysis of (4% PFA) fixed A375 cells using IGF2BP1 antibody (22803-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 22803-1-AP (IGF2BP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 22803-1-AP (IGF2BP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).