A549 cells were subjected to SDS PAGE followed by western blot with 17509-1-AP (IRS1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

IP result of anti-IRS1 (IP:17509-1-AP, 5ug; Detection:17509-1-AP 1:1000) with A549 cells lysate 3500ug.

Immunohistochemical analysis of paraffin-embedded human breast cancer using 17509-1-AP (IRS1 antibody) at dilution of 1:100 (under 40x lens).

Immunohistochemical analysis of paraffin-embedded human breast cancer using 17509-1-AP (IRS1 antibody) at dilution of 1:100 (under 10x lens).

Immunofluorescent analysis of (4% PFA) fixed A549 cells using IRS1 antibody (17509-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug Anti-Human IRS1 (17509-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

1X10^6 MCF-7 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human IRS1 (CL488-17509) (red), or 0.8 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Immunofluorescent analysis of (4% PFA) fixed A549 cells using CoraLite® Plus 488 IRS1 antibody (CL488-17509) at dilution of 1:200.