Various lysates were subjected to SDS PAGE followed by western blot with 12987-1-AP (Lamin B1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 12987-1-AP (Lamin B1 antibody) at dilution of 1:200 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12987-1-AP (Lamin B1 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

1X10^6 HEK-293 cells were stained with 0.2ug Lamin B1 antibody (12987-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). FITC-Goat anti-Rabbit IgG with dilution 1:100.

IP Result of anti-LMNB1 (IP:12987-1-AP, 3ug; Detection:12987-1-AP 1:1000) with HeLa cells lysate 2500ug.

Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Lamin B1 antibody (12987-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 66095-1-Ig (Lamin B1 antibody) at dilution of 1:500 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Immunofluorescent analysis of (4% PFA) fixed mouse eye tissue using Lamin B1 antibody (66095-1-Ig, Clone: 3C10G12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Western blot on multiple cells/tissues with anti-LMNB1 (66095-1-Ig) at dilution 1:20000.

Various lysates were subjected to SDS PAGE followed by western blot with 66095-1-Ig (Lamin B1 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66095-1-Ig (Lamin B1 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

1X10^6 HeLa cells were stained with 0.2ug Lamin B1 antibody (66095-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). FITC-Goat anti-Mouse IgG with dilution 1:100.

Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66095-1-Ig (Lamin B1 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

IP Result of anti-Lamin B1 (IP:66095-1-Ig, 4ug; Detection:66095-1-Ig 1:20000) with HeLa cells lysate 3560ug.

WB result of Lamin B1 antibody (66095-1-Ig; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Lamin B1 transfected HeLa cells.

Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite®594 Lamin B1 antibody (CL594-66095, Clone: 3C10G12 ) at dilution of 1:200.

1X10^6 HeLa cells were stained with 0.2 ug CoraLite®594 Anti-Human Lamin B1 (CL594-66095, Clone: 3C10G12) (red), or 0.2 ug CoraLite®594 Mouse IgG1 Isotype Control (CL594-66360, Clone: T1F8D3F10) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CL647-66095 (Lamin B1 antibody) at dilution of 1:100.

1X10^6 HeLa cells were stained with 0.2 ug CoraLite® Plus 647 Anti-Human Lamin B1 (CL647-66095, Clone: 3C10G12) (red), or 0.2 ug CoraLite® Plus 647 Mouse IgG1 Isotype Control (CL647-66360, Clone: T1F8D3F10) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CL488-66095 (Lamin B1 antibody) at dilution of 1:100.

1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®488 Anti-Human Lamin B1 (CL488-66095, Clone:3C10G12) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite®532 Lamin B1 antibody (CL532-66095, Clone: 3C10G12 ) at dilution of 1:400.

Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CoraLite®555 Lamin B1 antibody (CL555-66095, Clone: 3C10G12 ) at dilution of 1:800.

1X10^6 HeLa cells were intracellularly stained with 0.25 ug CoraLite®555 Anti-Human Lamin B1 (CL555-66095, Clone:3C10G12) (red), or 0.25 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using CoraLite®555 Lamin B1 antibody (CL555-66095, Clone: 3C10G12 ) at dilution of 1:1000.

Various lysates were subjected to SDS PAGE followed by western blot with 80906-1-RR (Lamin B1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

WB result of Lamin B1 antibody (80906-1-RR; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Lamin B1 transfected HeLa cells.

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 80906-1-RR (Lamin B1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Lamin B1 antibody (80906-1-RR, Clone: 5H10 ) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).