Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®488 Phospho-MAP2K3 (Ser189) antibody (CL488-80137, Clone: 3O10 ) at dilution of 1:200.

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Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®555 MAP2K3 antibody (CL555-80137, Clone: 3O10 ) at dilution of 1:200.

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Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®594 MAP2K3 antibody (CL594-80137, Clone: 3O10 ) at dilution of 1:200.

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1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human MAP2K3 (CL594-80137, Clone:3O10) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Various lysates were subjected to SDS PAGE followed by western blot with 68574-1-Ig (MAP2K3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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WB result of MAP2K3 antibody (68574-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MAP2K3 transfected A431 cells.

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Various lysates were subjected to SDS PAGE followed by western blot with 68574-1-Ig (MAP2K3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68574-1-PBS in a different storage buffer formulation.

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WB result of MAP2K3 antibody (68574-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MAP2K3 transfected A431 cells. This data was developed using the same antibody clone with 68574-1-PBS in a different storage buffer formulation.

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Various lysates were subjected to SDS PAGE followed by western blot with 80137-1-RR (MAP2K3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MAP2K3 antibody (80137-1-RR, Clone: 3O10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). DAPI (blue)

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WB result of MAP2K3 antibody (80137-1-RR; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MAP2K3 transfected HepG2 cells.

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Various lysates were subjected to SDS PAGE followed by western blot with 80137-1-RR (MAP2K3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80137-1-PBS in a different storage buffer formulation.

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Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MAP2K3 antibody (80137-1-RR, Clone: 3O10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). DAPI (blue) This data was developed using the same antibody clone with 80137-1-PBS in a different storage buffer formulation.

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WB result of MAP2K3 antibody (80137-1-RR; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MAP2K3 transfected HepG2 cells. This data was developed using the same antibody clone with 80137-1-PBS in a different storage buffer formulation.

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Non-treated HEK-293 cells, UV treated HEK-293 cells, non-treated HeLa cells and Anisomycin treated HeLa cells were subjected to SDS PAGE followed by western blot with 28903-1-AP (Phospho-MEK6 (Ser207) antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

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