Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 MEK6 antibody (CL488-12745) at dilution of 1:200.

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1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human MEK6 (CL488-12745) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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1X10^6 HeLa cells were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human MEK6 (CL647-12745) (red), or 0.2 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite®555 MEK6 antibody (CL555-12745) at dilution of 1:200.

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MAP2K6 fusion protein Ag31565 SDS-PAGE

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Non-treated HEK-293 cells, UV treated HEK-293 cells, non-treated HeLa cells and Anisomycin treated HeLa cells were subjected to SDS PAGE followed by western blot with 28903-1-AP (Phospho-MEK6 (Ser207) antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

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HeLa cells were subjected to SDS PAGE followed by western blot with 12745-1-AP (MEK6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12745-1-AP (MEK6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12745-1-AP (MEK6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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1X10^6 MCF-7 cells were stained with 0.2ug MEK6 antibody (12745-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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HEK-293 cells were subjected to SDS PAGE followed by western blot with 51094-1-AP (MEK6 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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MAP2K6 fusion protein Ag3706 SDS-PAGE

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