Various lysates were subjected to SDS PAGE followed by western blot with 16225-1-AP (MCL1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using MCL1 antibody (16225-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

IP result of anti-MCL1 (IP:16225-1-AP, 3ug; Detection:16225-1-AP 1:1000) with Raji cells lysate 800ug.

1X10^6 Ramos cells were intracellularly stained with 0.5 ug Anti-Human MCL1 (16225-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using KHC0434 (MCL1 IHC Kit).

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using KHC0434 (MCL1 IHC Kit).

Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using KHC0434 (MCL1 IHC Kit).

Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 15825-1-AP (MCL1L-specific antibody at dilution of 1:50 (under 10x lens).

Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 15825-1-AP (MCL1L-specific antibody at dilution of 1:50 (under 40x lens).

Non-treated and MG132 treated HeLa cells were subjected to SDS PAGE followed by western blot with 29560-1-AP (Phospho-MCL1 (Thr163) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as the loading control.