Various lysates were subjected to SDS PAGE followed by western blot with 67733-1-Ig (METTL3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 67733-1-PBS in a different storage buffer formulation.
WB result of METTL3 antibody (67733-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-METTL3 transfected A549 cells. This data was developed using the same antibody clone with 67733-1-PBS in a different storage buffer formulation.
WB result of METTL3 antibody (67733-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-METTL3 transfected A549 cells. This data was developed using the same antibody clone with 67733-1-PBS in a different storage buffer formulation.
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67733-1-Ig (METTL3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67733-1-PBS in a different storage buffer formulation.
Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using METTL3 antibody (67733-1-Ig, Clone: 2D8H1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67733-1-PBS in a different storage buffer formulation.
1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug Anti-Human METTL3 (67733-1-Ig, Clone:2D8H1) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 67733-1-PBS in a different storage buffer formulation.
