Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using SIK1 antibody (51045-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

A549 cells were subjected to SDS PAGE followed by western blot with 51045-1-AP (SIK1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

WB result of SIK1 antibody (51045-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SIK1 transfected A549 cells.

Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 51045-1-AP (SIK1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Various lysates were subjected to SDS PAGE followed by western blot with 82851-3-RR (Snf1lk antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82851-3-PBS in a different storage buffer formulation.

Various lysates were subjected to SDS PAGE followed by western blot with 82851-3-RR (Snf1lk antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

SIK1 fusion protein Ag28491 SDS-PAGE

Snf1lk fusion protein Ag27921 SDS-PAGE

Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 13880-1-AP (Aggrecan antibody at dilution of 1:400 (under 10x lens).

Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 13880-1-AP (Aggrecan antibody at dilution of 1:400 (under 40x lens).

1X10^6 ATDC-5 cells were intracellularly stained with 0.8 ug Anti-Human Aggrecan (13880-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.8 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 18309-1-AP (CD61 / Integrin Beta 3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

IP result of anti-CD61 / Integrin Beta 3 (IP:18309-1-AP, 4ug; Detection:18309-1-AP 1:300) with mouse spleen tissue lysate 5200 ug.

human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 18309-1-AP (CD61 / Integrin Beta 3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

IP result of anti-CD61 / Integrin Beta 3 (IP:18309-1-AP, 4ug; Detection:18309-1-AP 1:500) with mouse spleen tissue lysate 1920 ug.

Various lysates were subjected to SDS PAGE followed by western blot with 27096-1-AP (Integrin Alpha V antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

Immunofluorescent analysis of (4% PFA) fixed A549 cells using Integrin Alpha V antibody (27096-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 27096-1-AP (Integrin Alpha V antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 27096-1-AP (Integrin Alpha V antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).