Jurkat cells were subjected to SDS PAGE followed by western blot with 22023-1-AP (NFATC2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22023-1-AP (NFATC2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IP result of anti-NFATC2 (IP:22023-1-AP, 4ug; Detection:22023-1-AP 1:1000) with Jurkat cells lysate 3200ug.
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22023-1-AP (NFATC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human NFATC2 (22023-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
Immunofluorescent analysis of (4% PFA) fixed U-251 cells using NFATC2 antibody (22023-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).