1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD81 (65195-1-Ig, Clone:5A6) and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 or unstained. Cells were not fixed. Lymphocytes were gated.

1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD81 (65195-1-Ig, Clone:5A6) and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 or unstained. Cells were not fixed. Lymphocytes were gated.

1X10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 488 Anti-Human CD81 (CL488-65195, Clone:5A6) (red), or Mouse IgG1 Isotype Control (blue). Cells were not fixed. Lymphocytes were gated.

1X10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 488 Anti-Human CD81 (CL488-65195, Clone:5A6), or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

1X10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 647 Anti-Human CD81 (CL647-65195, Clone:5A6) (red) or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

1X10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 647 Anti-Human CD81 (CL647-65195, Clone:5A6) or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

Purity of recombinant human BMP-4 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

Recombinant human BMP-4 (HZ-1045) stimulates dose-dependent induction of alkaline phosphatase production in the ATDC-5 mouse chondrogenic cell line. Alkaline phosphatase production was assessed using pNPP as a chromogenic substrate. ATDC-5 cells were treated with increasing concentrations of recombinant human BMP-4 for 72 hours before lysis and addition of pNPP. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 values range from 1.5-9 ng/ml.

Proteintech BMP-4 (HZ-1045) demonstrates equivalent induction and 2-fold lower EC50 compared to leading competitors. Recombinant human BMP-4 stimulates dose-dependent induction of alkaline phosphatase production in the ATDC-5 mouse chondrogenic cell line. Alkaline phosphatase production was assessed using pNPP as a chromogenic substrate. ATDC-5 cells were treated with increasing concentrations of recombinant human BMP-4 for 72 hours before lysis and addition of pNPP. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 values range from 1.5-9 ng/ml.

Human iPSC-derived microglia (white) residing within an in vivo brain-like organoid environment (blue) derived using HumanKine growth factors (TGF beta 1- HZ-1011, BMP4- HZ-1045, and Thrombopoietin HZ-1248).(Credits- Credit: Simon T. Schafer & Monique Pena, Technical University of Munich, Center for Organoid Systems)

Human induced pluripotent cells(iPSCs) were differentiated to definitive endoderm using HumanKine growth factors (Activin A- HZ-1138, bFGF-HZ-1285, BMP-4- HZ-1045, and Wnt3A-HZ-1296). The differentiation was confirmed by detection of expression of FOXA2(Red) and downregulation of pluripotency marker NANOG(yellow), in immunofluorescence

Purity of recombinant human FGFbasic-TS was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

Recombinant human FGFbasic-TS (HZ-1285) stimulates dose-dependent proliferation of the HDFa human primary fibroblast cell line. Cell number was quantitatively assessed by Promega CellTiter 96® cell viability reagent. HDFa cells were treated with increasing concentrations of recombinant FGFbasic-TS for 48 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 0.05-0.4 ng/mL.

Recombinant human FGFbasic-TS (HZ-1285) stimulates dose-dependent proliferation of the NIH/3T3 mouse fibroblast cell line. Viable cell number was quantitatively assessed by Prestoblue Cell Viability Reagent. NIH/3T3 cells were serum starved with 0.02% FBS during treatment with increasing concentrations of recombinant human FGF basic-TS for 72hrs in defined medium. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 values range from 0.4-2.5 ng/mL.

Proteintech FGF basic-TS (HZ-1285) demonstrates greater induction of proliferation and 3-fold lower EC50 compared to leading competitors. Recombinant human FGFbasic-TS stimulates dose-dependent proliferation of the HDFa human primary fibroblast cell line. Cell number was quantitatively assessed by Promega CellTiter 96® cell viability reagent. HDFa cells were treated with increasing concentrations of recombinant FGFbasic-TS for 48 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 0.05-0.4 ng/mL.

Human induced pluripotent cells(iPSCs) were differentiated to definitive endoderm using HumanKine growth factors (Activin A- HZ-1138, bFGF-HZ-1285, BMP-4- HZ-1045, and Wnt3A-HZ-1296). The differentiation was confirmed by detection of expression of FOXA2(Red) and downregulation of pluripotency marker NANOG(yellow), in immunofluorescence

Purity of recombinant human IL-2 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

recombinant human IL-2 (HZ-1015) stimulates dose-dependent proliferation of the CTLL-2 (mouse cytotoxic T) cell line. Viable cell number was quantitatively assessed by Prestoblue Cell Viability Reagent. CTLL-2 cells were starved for 5 hours before treatment with increasing concentrations of GMP recombinant human IL-2 for 48 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 values range from 0.05-0.35 ng/mL.

Purity of recombinant human SCF was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue

Recombinant human SCF (HZ-1024) stimulates dose-dependent proliferation of the MO7e human megakaryoblastic leukemia cell line. Cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. MO7e cells were treated with increasing concentrations of GMP recombinant SCF for 72 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 15-85 ng/mL​.

Purity of recombinant human FLT3 Ligand was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

Recombinant human FLT3 (HZ-1151) Ligand stimulates dose-dependent proliferation of the human acute myeloid leukemia cell line (OCI-AML5). Viable cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. OCI-AML5 cells were treated with increasing concentrations of recombinant human FLT3 Ligand for 72 hours. The EC50 was determined using a 4- parameter non-linear regression model. The EC50 values range is 0.4-3 ng/mL.