Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PCNA antibody (10205-2-AP) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL94-phalloidin (red), DAPI (Blue).
1X10^6 Jurkat cells were intracellularly stained with 0.2 ug Anti-Human PCNA (10205-2-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), and 0.2 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).
WB result of PCNA antibody (10205-2-AP, 1:10000) with si-control and si-PCNA transfected HEK293 cells.
IP Result of anti-PCNA (IP:10205-2-AP, 3ug; Detection:10205-2-AP 1:1500) with MCF-7 cells lysate 2500ug.
Immunohistochemical analysis of paraffin-embedded human stomach cancer using 10205-2-AP (PCNA antibody) at dilution of 1:500 (under 40x lens).
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 10205-2-AP (PCNA antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Various lysates were subjected to SDS PAGE followed by western blot with 10205-2-AP (PCNA antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 10205-2-AP (PCNA antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10205-2-AP (PCNA antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using PCNA antibody (10205-2-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using PCNA antibody (10205-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).