Various lysates were subjected to SDS PAGE followed by western blot with 18262-1-AP (PDK1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18262-1-AP (PDK1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using PDK1 antibody (18262-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using KHC0190 (PDK1 IHC Kit).

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using KHC0190 (PDK1 IHC Kit).

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using KHC0190 (PDK1 IHC Kit).

MCF-7 cells were subjected to SDS PAGE followed by western blot with 17086-1-AP (PDPK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17086-1-AP (PDPK1 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17086-1-AP (PDPK1 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using KHC1448 (PDPK1 IHC Kit).

Immunohistochemical analysis of paraffin-embedded human lung tissue slide using KHC1448 (PDPK1 IHC Kit).

Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using KHC1448 (PDPK1 IHC Kit).

Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using KHC1448 (PDPK1 IHC Kit).

Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using KHC1448 (PDPK1 IHC Kit).

Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using KHC1448 (PDPK1 IHC Kit).

PDK1 fusion protein Ag23160 SDS-PAGE

Non-treated PC-3 and PDGF (HZ-1215) treated PC-3 cells were subjected to SDS PAGE followed by western blot with 29241-1-AP (Phospho-PDPK1 (Ser241) antibody) at dilution of 1:1000 incubated at 4℃ overnight.

Non-treated NIH/3T3 and PDGF (HZ-1215) treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 29241-1-AP (Phospho-PDPK1 (Ser241) antibody) at dilution of 1:2000 incubated at 4℃ overnight. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

PDPK1 fusion protein Ag9213 SDS-PAGE