Various lysates were subjected to SDS PAGE followed by western blot with 55028-1-AP (PFKM antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB result of PFKM antibody (55028-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PFKM transfected HeLa cells.
WB result of PFKM antibody (55028-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PFKM transfected HEK-293 cells.
Immunofluorescent analysis of HeLa cells using 55028-1-AP (PFKM antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.
IP result of anti-PFKM (IP:55028-1-AP, 4ug; Detection:55028-1-AP 1:8000) with mouse heart tissue lysate 1680 ug.
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 55028-1-AP (PFKM antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 55028-1-AP (PFKM antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).