rat liver tissue were subjected to SDS PAGE followed by western blot with 20584-1-AP (PI3 Kinase p110 Beta antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 20584-1-AP (PI3 Kinase p110 Beta antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

IP result of anti-PI3 Kinase p110 Beta (IP:20584-1-AP, 4ug; Detection:20584-1-AP 1:300) with HepG2 cells lysate 2700ug.

1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human PI3 Kinase p110 Beta (20584-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH.

U2OS cells were subjected to SDS PAGE followed by western blot with 67071-1-Ig (PI3 Kinase p110 Alpha antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control.

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67071-1-Ig (PIK3CA antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67071-1-Ig (PIK3CA antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using PI3 Kinase p110 Alpha antibody (67071-1-Ig, Clone: 1F6A7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human PI3 Kinase p110 Alpha (67071-1-Ig, Clone:1F6A7) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (MPC-11) (65128-1-Ig, Clone: MPC-11) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Various lysates were subjected to SDS PAGE followed by western blot with 67121-1-Ig (PIK3CB antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67121-1-Ig (PIK3CB antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67121-1-Ig (PIK3CB antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Various lysates were subjected to SDS PAGE followed by western blot with 27921-1-AP (PI3K p110(alpha) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Various lysates were subjected to SDS PAGE followed by western blot with 27921-1-AP (PI3 Kinase p110 Alpha antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

Various lysates were subjected to SDS PAGE followed by western blot with 20662-1-AP (PI3 Kinase p110 Gamma antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

IP Result of anti-PI3K p110(gamma) (IP:20662-1-AP, 5ug; Detection:20662-1-AP 1:300) with mouse liver tissue lysate 4000ug.

Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 20662-1-AP (PI3 Kinase p110 Gamma antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with .

Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 20662-1-AP (PI3 Kinase p110 Gamma antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with .

Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®488 PI3 Kinase p110 Beta antibody (CL488-67121, Clone: 1B2A1 ) at dilution of 1:200.

Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®488 PI3 Kinase p110 Beta antibody (CL488-20584) at dilution of 1:100.

1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®488 Anti-Human PI3 Kinase p110 Beta (CL488-20584) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

1X10^6 HeLa cells were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human PI3 Kinase p110 Beta (CL647-20584) (red), or 0.2 ug isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).