Various lysates were subjected to SDS PAGE followed by western blot with 27398-1-AP (PRKACA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB result of PRKACA antibody (27398-1-AP; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-PRKACA transfected HeLa cells.
IP result of anti-PRKACA (IP:27398-1-AP, 4ug; Detection:27398-1-AP 1:300) with HeLa cells lysate 2600 ug.
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 27398-1-AP (PRKACA antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 27398-1-AP (PRKACA antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunofluorescent analysis of (4% PFA) fixed U2OS cells using PRKACA antibody (27398-1-AP) at dilution of 1:200 and Cy3-conjugated Affinipure Goat Anti-Rabbit IgG(H+L).