Various lysates were subjected to SDS PAGE followed by western blot with 21991-1-AP (PKC alpha antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Various lysates were subjected to SDS PAGE followed by western blot with 21991-1-AP (PKC Alpha antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

IP result of anti-PKC Alpha (IP:21991-1-AP, 4ug; Detection:21991-1-AP 1:3000) with NIH/3T3 cells lysate 1320 ug.

Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 21991-1-AP (PKC alpha antibody at dilution of 1:200 (under 10x lens).

Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 21991-1-AP (PKC alpha antibody at dilution of 1:200 (under 40x lens).

Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PKC Alpha antibody (21991-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human PKC Alpha (21991-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PKC Alpha antibody (21991-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Various lysates were subjected to SDS PAGE followed by western blot with 12919-1-AP (PKC Beta antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12919-1-AP (PKC beta antibody) at dilution of 1:200 (under 10x lens).

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12919-1-AP (PKC beta antibody) at dilution of 1:200 (under 40x lens).

IP result of anti-pan-PKC (IP:12919-1-AP, 4ug; Detection:12919-1-AP 1:1000) with mouse brain tissue lysate 4000 ug.

1X10^6 K-562 cells were intracellularly stained with 0.4 ug Anti-Human PKC Beta (12919-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Immunofluorescence analysis of bipolar cells labelled with pan-PKC polyclonal antibody (12919-1-AP, green) on tissue sections of the zebrafish retina at 5 days post fertilisation. Dilution 1:300. Tissue fixed overnight in 4% PFA. Müller glia, red; DAPI, blue; HuC/D, magenta. Data generated by Natalia Jaroszynska in Professor Ryan MacDonald's lab, University College London, UK.

Various lysates were subjected to SDS PAGE followed by western blot with 20877-1-AP (PKC Epsilon antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20877-1-AP (PKC epsilon antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20877-1-AP (PKC epsilon antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PKC Epsilon antibody (20877-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using PKC Epsilon antibody (20877-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using PKC Epsilon antibody (20877-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using PKC Epsilon antibody (20877-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

IP result of anti-PKC Epsilon(IP:20877-1-AP, 4ug; Detection:20877-1-AP 1:800) with SH-SY5Y cells lysate 1240 ug.

1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human PKC Epsilon (20877-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Various lysates were subjected to SDS PAGE followed by western blot with 14188-1-AP (PKC Delta antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

IP result of anti-PKC Delta(IP:14188-1-AP, 4ug; Detection:14188-1-AP 1:2000) with mouse brain tissue lysate 1280 ug.

Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PKC Delta antibody (14188-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

Immunofluorescent analysis of (4% PFA) fixed Saos-2 cells using PKC Delta antibody (14188-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

mouse brain tissue were subjected to SDS PAGE followed by western blot with 14364-1-AP (PKC gamma antibody at dilution of 1:10000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 14364-1-AP (PKC gamma antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 14364-1-AP (PKC gamma antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 14364-1-AP (PKC gamma antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

IP Result of anti-PKC gamma (IP:14364-1-AP, 4ug; Detection:14364-1-AP 1:500) with mouse brain tissue lysate 2640ug.

1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human PKC Gamma (14364-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Various lysates were subjected to SDS PAGE followed by western blot with 19132-1-AP (PKC Delta antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 19132-1-AP (PKC delta antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 19132-1-AP (PKC delta antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

IP result of anti-PKC Delta(IP:19132-1-AP, 4ug; Detection:19132-1-AP 1:800) with A431 cells lysate 2640 ug.

IP Result of anti-PKC delta (IP:19132-1-AP, 4ug; Detection:19132-1-AP 1:500) with A431 cells lysate 2000ug.

Immunofluorescent analysis of (4% PFA) fixed U2OS cells using PKC Delta antibody (19132-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human PKC Delta (19132-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Immunofluorescent analysis of (4% PFA) fixed U-251 cells using PKC Delta antibody (19132-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PKC Delta antibody (19132-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

Non-treated and PMA treated THP-1 cells were subjected to SDS PAGE followed by western blot with 29564-1-AP (Phospho-PKC delta (Thr507) antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with PKC Delta antibody (19132-1-AP) subsequently.

Various lysates were subjected to SDS PAGE followed by western blot with 13883-1-AP (PKC Iota antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

IP result of anti-PKC Iota (IP:13883-1-AP, 4ug; Detection:13883-1-AP 1:2000) with HeLa cells lysate 1720 ug.

WB result of PKC Iota antibody (13883-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PKC Iota transfected MCF-7 cells.

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13883-1-AP (PKC iota antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13883-1-AP (PKC iota antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using PKC Iota antibody (13883-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human PKC Iota (13883-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Non-treated NIH/3T3, phosphatase inhibitor treated and λ phosphatase treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 29123-1-AP (Phospho-PKC Alpha (Thr638) antibody) at dilution of 1:5000 incubated at room temperature for 1 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

Non-treated and PMA treated HL-60 cells were subjected to SDS PAGE followed by western blot with 29123-1-AP (Phospho-PKC Alpha (Thr638) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

Immunofluorescent analysis of (-20°C Ethanol) fixed λ phosphatase treated HeLa cells using Phospho-PKC Alpha (Thr638) antibody (29123-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).