COLO 320 cells were subjected to SDS PAGE followed by western blot with 19675-1-AP (HSPG2-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

HSPG2 fusion protein Ag30846 SDS-PAGE

Various lysates were subjected to SDS PAGE followed by western blot with 15967-1-AP (ERp57/ERp60 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

IP result of anti-ERp57/ERp60 (IP:15967-1-AP, 4ug; Detection:15967-1-AP 1:2000) with mouse liver tissue lysate 4000ug.

Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ERp57/ERp60 antibody (15967-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15967-1-AP (ERp57/ERp60 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15967-1-AP (ERp57/ERp60 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

1X10^6 HepG2 cells were stained with 0.2ug ERp57/ERp60 antibody (15967-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using CL488-66423 (PDIA3 antibody) at dilution of 1:100.

Various lysates were subjected to SDS PAGE followed by western blot with 66423-1-Ig (ERp57/ERp60 antibody) at dilution of 1:9700 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

Immunofluorescent analysis of (-20°C Methanol) fixed HEK-293 cells using ERp57/ERp60 antibody (66423-1-Ig, Clone: 3G4G7 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66423-1-Ig (ERp57/ERp60 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66423-1-Ig (ERp57/ERp60 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Various lysates were subjected to SDS PAGE followed by western blot with 66423-1-Ig (ERp57/ERp60 antibody) at dilution of 1:9700 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66423-1-PBS in a different storage buffer formulation.

Immunofluorescent analysis of (-20°C Methanol) fixed HEK-293 cells using ERp57/ERp60 antibody (66423-1-Ig, Clone: 3G4G7 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66423-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66423-1-Ig (ERp57/ERp60 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66423-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66423-1-Ig (ERp57/ERp60 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66423-1-PBS in a different storage buffer formulation.

mouse brain tissue were subjected to SDS PAGE followed by western blot with 26551-1-AP (Phospholipase C beta 1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 26551-1-AP (Phospholipase C beta 1 antibody at dilution of 1:200 (under 10x lens).

Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 26551-1-AP (Phospholipase C beta 1 antibody at dilution of 1:200 (under 40x lens).

Various lysates were subjected to SDS PAGE followed by western blot with 66668-1-Ig (PLCB3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control.

Various lysates were subjected to SDS PAGE followed by western blot with 66668-1-Ig (PLCB3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Various lysates were subjected to SDS PAGE followed by western blot with 66668-1-Ig (PLCB3 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

Sp2/0 cells were subjected to SDS PAGE followed by western blot with 66668-1-Ig (PLCB3 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66668-1-Ig (PLCB3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66668-1-Ig (PLCB3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).