Various lysates were subjected to SDS PAGE followed by western blot with 66592-1-Ig (RAF1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66592-1-Ig (RAF1 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66592-1-Ig (RAF1 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

WB result of RAF1 antibody (66592-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RAF1 transfected HEK-293 cells.

Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using RAF1 antibody (66592-1-Ig, Clone: 1D6A1 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). DAPI (Blue).

DU 145 cells were subjected to SDS PAGE followed by western blot with 26863-1-AP (RAF1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 26863-1-AP (RAF1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 26863-1-AP (RAF1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using CoraLite®®594 RAF1 antibody (CL594-66592, Clone: 1D6A1 ) at dilution of 1:200.

Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using KHC1506 (RAF1 IHC Kit).

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using KHC1506 (RAF1 IHC Kit).

Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using KHC1506 (RAF1 IHC Kit).

Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using KHC1506 (RAF1 IHC Kit).

Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using KHC1506 (RAF1 IHC Kit).

Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using KHC1506 (RAF1 IHC Kit).

Various lysates were subjected to SDS PAGE followed by western blot with 66592-1-Ig (RAF1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66592-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66592-1-Ig (RAF1 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66592-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66592-1-Ig (RAF1 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66592-1-PBS in a different storage buffer formulation.

WB result of RAF1 antibody (66592-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RAF1 transfected HEK-293 cells. This data was developed using the same antibody clone with 66592-1-PBS in a different storage buffer formulation.

Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using RAF1 antibody (66592-1-Ig, Clone: 1D6A1 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). DAPI (Blue). This data was developed using the same antibody clone with 66592-1-PBS in a different storage buffer formulation.

RAF1 fusion protein Ag25402 SDS-PAGE

RAF1 fusion protein Ag25423 SDS-PAGE

Various lysates were subjected to SDS PAGE followed by western blot with 20899-1-AP (BRAF antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using 20899-1-AP (BRAF antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 20899-1-AP (BRAF Antibody) at dilution of 1:200 (under 10x lens).

Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 20899-1-AP (BRAF Antibody) at dilution of 1:200 (under 40x lens).