Various lysates were subjected to SDS PAGE followed by western blot with 22167-1-AP (IQGAP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22167-1-AP (IQGAP1 Antibody) at dilution of 1:200 (under 10x lens).
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22167-1-AP (IQGAP1 Antibody) at dilution of 1:200 (under 40x lens).
IP result of anti-IQGAP1(IP:22167-1-AP, 4ug; Detection:22167-1-AP 1:8000) with HeLa cells lysate 1280 ug.
Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using IQGAP1 antibody (22167-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using IQGAP1 antibody (22167-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 22167-1-AP (IQGAP1 antibody) at dilution of 1:400 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human IQGAP1 (22167-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).