Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using KHC2057 (RASGRF2 IHC Kit).

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rat brain tissue were subjected to SDS PAGE followed by western blot with 12958-1-AP (RASGRF1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 12958-1-AP (RASGRF1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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IP result of anti-RASGRF1(IP:12958-1-AP, 4ug; Detection:12958-1-AP 1:600) with rat brain tissue lysate 1120 ug.

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Non-treated and Forskolin treated HeLa cells were subjected to SDS PAGE followed by western blot with 82309-1-RR (Phospho-RASGRF1 (Ser927) antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with mTOR (66888-1-Ig) as loading control.

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Non-treated and Forskolin treated HeLa cells were subjected to SDS PAGE followed by western blot with 82309-1-RR (Phospho-RASGRF1 (Ser927) antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with mTOR (66888-1-Ig) as loading control. This data was developed using the same antibody clone with 82309-1-PBS in a different storage buffer formulation.

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pig cerebellum tissue were subjected to SDS PAGE followed by western blot with 67010-1-Ig (RASGRF1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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pig cerebellum tissue were subjected to SDS PAGE followed by western blot with 67010-1-Ig (RASGRF1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67010-1-PBS in a different storage buffer formulation.

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Various lysates were subjected to SDS PAGE followed by western blot with 26788-1-AP (RASGRF2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 26788-1-AP (RASGRF2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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