Various lysates were subjected to SDS PAGE followed by western blot with 26997-1-AP (RASGRP1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using RASGRP1 antibody (26997-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

RASGRP1 fusion protein Ag25725 SDS-PAGE

RASGRP1 fusion protein Ag25736 SDS-PAGE

Various lysates were subjected to SDS PAGE followed by western blot with 13162-1-AP (RASGRP3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

WB result of RASGRP3 antibody (13162-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RASGRP3 transfected PC-3 cells.

IP result of anti-RASGRP3 (IP:13162-1-AP, 4ug; Detection:13162-1-AP 1:500) with RAW 264.7 cells lysate 3200 ug.

1x10^6 Jurkat cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Rasgrp2 Polyclonal Antibody (CL488-30189)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using CoraLite®594 RASGRP3 antibody (CL594-66650, Clone: 1A10G4 ) at dilution of 1:200.

Various lysates were subjected to SDS PAGE followed by western blot with 30189-1-AP (RASGRP2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human RASGRP2 (30189-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Various lysates were subjected to SDS PAGE followed by western blot with 66650-1-Ig (RASGRP3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using RASGRP3 antibody (66650-1-Ig, Clone: 1A10G4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).