A431 cells were subjected to SDS PAGE followed by western blot with 10048-2-Ig (RB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
IP result of anti-RB1 (IP:10048-2-Ig, 3ug; Detection:10048-2-Ig 1:500) with A431 cells lysate 3000 ug.
Non-treated and Calyculin A treated Jurkat cells were subjected to SDS PAGE followed by western blot with 30376-1-AP (Phospho-RB1 (Ser807/811) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with RB1 antibody (10048-2-Ig) subsequently.
HT-1080 cells were subjected to SDS PAGE followed by western blot with 17218-1-AP (RB1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
Non-treated and Calyculin A treated Jurkat cells were subjected to SDS PAGE followed by western blot with 30376-1-AP (Phospho-RB1 (Ser807/811) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with RB1 antibody (10048-2-Ig) subsequently.
Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using CoraLite® Plus 488 RB1 antibody (CL488-67521, Clone: 1A2A6 ) at dilution of 1:200.
1X10^6 Jurkat cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human RB1 (CL488-67521, Clone:1A2A6) (red), or 0.4 ug CoraLite® Plus 488 Mouse IgG2a Isotype Control (C1.18.4) (CL488-65208, Clone: C1.18.4) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.
Jurkat cells were subjected to SDS PAGE followed by western blot with 67521-1-Ig (RB1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
Immunohistochemical analysis of paraffin-embedded Insulinoma tissue slide using 67521-1-Ig (RB1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using RB1 antibody (67521-1-Ig, Clone: 1A2A6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
Jurkat cells were subjected to SDS PAGE followed by western blot with 67521-1-Ig (RB1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67521-1-PBS in a different storage buffer formulation.
Immunohistochemical analysis of paraffin-embedded Insulinoma tissue slide using 67521-1-Ig (RB1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67521-1-PBS in a different storage buffer formulation.
Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using RB1 antibody (67521-1-Ig, Clone: 1A2A6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67521-1-PBS in a different storage buffer formulation.
Various lysates were subjected to SDS PAGE followed by western blot with 30373-1-AP (RB1 antibody) at dilution of 1:25000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 30374-1-AP (RB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
