Non-treated Hela and Cobalt Chloride treated Hela cells were subjected to SDS PAGE followed by western blot with 10638-1-AP (REDD1 specific antibody) at dilution of 1:600 incubated at room temperature for 6 hours. .
WB result of REDD1 antibody (10638-1-AP, 1:1000) with si-control and si-REDD1 transfected PC-3 cells.
Various lysates were subjected to SDS PAGE followed by western blot with 10638-1-AP (REDD1 specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 10638-1-AP (REDD1 specific antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
IP result of anti-REDD1 specific (IP:10638-1-AP, 3ug; Detection:10638-1-AP 1:500) with MCF-7 cells lysate 2500ug.
Various lysates were subjected to SDS PAGE followed by western blot with 10638-1-AP (REDD1 specific antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10638-1-AP (REDD1 specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10638-1-AP (REDD1 specific antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10638-1-AP (REDD1 specific antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunofluorescent analysis of (-20°C Methanol) fixed LNCaP cells using REDD1 specific antibody (10638-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).