Various lysates were subjected to SDS PAGE followed by western blot with 66733-1-Ig (RHOA antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66733-1-PBS in a different storage buffer formulation.
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66733-1-Ig (RHOA antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66733-1-PBS in a different storage buffer formulation.
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66733-1-Ig (RHOA antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66733-1-PBS in a different storage buffer formulation.
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 66733-1-Ig (RHOA antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66733-1-PBS in a different storage buffer formulation.