Non-treated HeLa cells and Calyculin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 28983-1-AP (Phospho-RPS6KA1 (Ser380) antibody) at dilution of 1:1000 incubated at 4℃ overnight. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

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Non-treated NIH/3T3, phosphatase inhibitor treated and λ phosphatase treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 29225-1-AP (Phospho-RPS6KA1 (Thr359/Ser363) antibody) at dilution of 1:3000 incubated at 4℃ overnight.

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Non-treated NIH/3T3 and PDGF (HZ-1215) treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 29225-1-AP (Phospho-RPS6KA1 (Thr359/Ser363) antibody) at dilution of 1:2000 incubated at 4℃ overnight. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

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1X10^6 Jurkat cells untreated (dashed lines) or treated with TPA (red) were intracellularly stained with 0.13 ug CoraLite®488 Anti-Human Phospho-RPS6KA1 (Ser380) (CL488-80108, Clone:7F23), or 0.13 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

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1X10^6 Jurkat cells untreated (dashed lines) or treated with TPA (red) were intracellularly stained with 0.13 ug CoraLite®647 Anti-Human Phospho-RPS6KA1 (Ser380) (CL647-80108, Clone:7F23) (red), or 0.13 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

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Immunohistochemical analysis of paraffin-embedded Jurkat (left) and calyculin A treated Jurkat (right) cells slide using KHC0292 (Phospho-RPS6KA1 (Ser380) IHC Kit).

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Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using KHC0292 (Phospho-RPS6KA1 (Ser380) IHC Kit).

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Non-treated NIH/3T3 and PDGF (HZ-1215) treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 80108-1-RR (Phospho-RPS6KA1 (Ser380) antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

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1X10^6 Jurkat cells untreated (dashed line) or treated with TPA treated (red) were intracellularly stained with 0.4 ug Anti-Human Phospho-RPS6KA1 (Ser380) (80108-1-RR, Clone:7F23) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

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Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 80108-1-RR (Phospho-RPS6KA1 (Ser380) antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Non-treated NIH/3T3 and PDGF (HZ-1215) treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 80108-1-RR (Phospho-RPS6KA1 (Ser380) antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control. This data was developed using the same antibody clone with 80108-1-PBS in a different storage buffer formulation.

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1X10^6 Jurkat cells untreated (dashed line) or treated with TPA treated (red) were intracellularly stained with 0.4 ug Anti-Human Phospho-RPS6KA1 (Ser380) (80108-1-RR, Clone:7F23) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 80108-1-PBS in a different storage buffer formulation.

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Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 80108-1-RR (Phospho-RPS6KA1 (Ser380) antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80108-1-PBS in a different storage buffer formulation.

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Non-treated and λ phosphatase treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 68041-1-Ig (Phospho-RPS6KA1 (Thr359/Ser363) antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta-Tubulin (HRP-66240) as loading control.

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Immunofluorescent analysis of (4% PFA) fixed λ phosphatase treated HeLa cells using Phospho-RPS6KA1 (Thr359/Ser363) antibody (68041-1-Ig, Clone: 2C11D9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

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