WB result of RSK2 antibody (23762-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RSK2 transfected K-562 cells.

NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 23762-1-AP (RSK2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

IP result of anti-RSK2 (IP:23762-1-AP, 4ug; Detection:23762-1-AP 1:4000) with K-562 cells lysate 3680 ug.

Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 23762-1-AP (RSK2 antibody at dilution of 1:50(under 10x lens).

Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 23762-1-AP (RSK2 antibody at dilution of 1:50(under 40x lens).

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14446-1-AP (RSK3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 14446-1-AP (RSK3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

IP Result of anti-RSK3 (IP:14446-1-AP, 4ug; Detection:14446-1-AP 1:800) with HeLa cells lysate 4000ug.

Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 14446-1-AP (RSK3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Non-treated HeLa cells and Calyculin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 28983-1-AP (Phospho-RPS6KA1 (Ser380) antibody) at dilution of 1:1000 incubated at 4℃ overnight. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

Non-treated NIH/3T3, phosphatase inhibitor treated and λ phosphatase treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 29225-1-AP (Phospho-RPS6KA1 (Thr359/Ser363) antibody) at dilution of 1:3000 incubated at 4℃ overnight.

Non-treated NIH/3T3 and PDGF (HZ-1215) treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 29225-1-AP (Phospho-RPS6KA1 (Thr359/Ser363) antibody) at dilution of 1:2000 incubated at 4℃ overnight. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

1X10^6 Jurkat cells untreated (dashed lines) or treated with TPA (red) were intracellularly stained with 0.13 ug CoraLite®488 Anti-Human Phospho-RPS6KA1 (Ser380) (CL488-80108, Clone:7F23), or 0.13 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

1X10^6 Jurkat cells untreated (dashed lines) or treated with TPA (red) were intracellularly stained with 0.13 ug CoraLite®647 Anti-Human Phospho-RPS6KA1 (Ser380) (CL647-80108, Clone:7F23) (red), or 0.13 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

Immunohistochemical analysis of paraffin-embedded Jurkat (left) and calyculin A treated Jurkat (right) cells slide using KHC0292 (Phospho-RPS6KA1 (Ser380) IHC Kit).

Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using KHC0292 (Phospho-RPS6KA1 (Ser380) IHC Kit).

Non-treated NIH/3T3 and PDGF (HZ-1215) treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 80108-1-RR (Phospho-RPS6KA1 (Ser380) antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

1X10^6 Jurkat cells untreated (dashed line) or treated with TPA treated (red) were intracellularly stained with 0.4 ug Anti-Human Phospho-RPS6KA1 (Ser380) (80108-1-RR, Clone:7F23) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 80108-1-RR (Phospho-RPS6KA1 (Ser380) antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).