WB result of Raptor antibody (20984-1-AP, 1:500) with si-control and si-Raptor transfected HepG2 cells.

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Various lysates were subjected to SDS PAGE followed by western blot with 20984-1-AP (Raptor antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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IP result of anti-Raptor (IP:20984-1-AP, 4ug; Detection:20984-1-AP 1:4000) with HEK-293 cells lysate 1400 ug.

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Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using Raptor antibody (20984-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Various lysates were subjected to SDS PAGE followed by western blot with 68286-1-Ig (Raptor antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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IP result of anti-Raptor(IP:68286-1-Ig, 4ug; Detection:68286-1-Ig 1:1000) with HeLa cells lysate 1800 ug.

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Various lysates were subjected to SDS PAGE followed by western blot with 68286-1-Ig (Raptor antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68286-1-PBS in a different storage buffer formulation.

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IP result of anti-Raptor (IP:68286-1-Ig, 4ug; Detection:68286-1-Ig 1:1000) with HeLa cells lysate 1800 ug. This data was developed using the same antibody clone with 68286-1-PBS in a different storage buffer formulation.

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KIAA1303 fusion protein Ag30131 SDS-PAGE

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