Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25027-1-AP (RELB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IP result of anti-RELB (IP:25027-1-AP, 3ug; Detection:25027-1-AP 1:300) with Raji cells lysate 2500ug.
Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 25027-1-AP (RELB Antibody) at dilution of 1:50 (under 40x lens).
Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using RELB antibody (25027-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
1X10^6 Jurkat cells were intracellularly stained with 0.5 ug Anti-Human RELB (25027-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
Various lysates were subjected to SDS PAGE followed by western blot with 25027-1-AP (RELB antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
Immunofluorescent analysis of HeLa cells using 25027-1-AP (RELB antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.