Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using KHC0058 (P62,SQSTM1 IHC Kit).

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using KHC0058 (P62,SQSTM1 IHC Kit).

Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using KHC0058 (P62,SQSTM1 IHC Kit).

Various lysates were subjected to SDS PAGE followed by western blot with 18420-1-AP (P62,SQSTM1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

IP result of anti-P62,SQSTM1 (IP:18420-1-AP, 4ug; Detection:18420-1-AP 1:1000) with HEK-293 cells lysate 4000 ug.

WB result of P62,SQSTM1 antibody (18420-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-P62/SQSTM1 transfected HEK-293 cells.

Immunofluorescent analysis of (-20℃ Ethanol) fixed Starvation treated HepG2 cells using 18420-1-AP (P62,SQSTM1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunohistochemical analysis of paraffin-embedded human gliomas using 18420-1-AP (SQSTM1 antibody) at dilution of 1:50 (under 40x lens).

1X10^6 HEK-293T cells were stained with 0.2ug P62,SQSTM1 antibody (18420-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

Various lysates were subjected to SDS PAGE followed by western blot with 18420-1-AP (P62,SQSTM1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 18420-1-AP (P62,SQSTM1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18420-1-AP (P62,SQSTM1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18420-1-AP (P62,SQSTM1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of (-20°C Ethanol) fixed Chloroquine treated HeLa cells using P62,SQSTM1 antibody (18420-1-AP) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), (CL594-Phalloidin, red).

Immunofluorescent analysis of (-20°C Ethanol) fixed Chloroquine treated HepG2 cells using P62,SQSTM1 antibody (18420-1-AP) at dilution of 1:1500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

Various lysates were subjected to SDS PAGE followed by western blot with 66184-1-Ig (P62,SQSTM1 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

WB result of P62,SQSTM1 antibody (66184-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-P62/SQSTM1 transfected HEK-293 cells.

Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using P62/SQSTM1 antibody (66184-1-Ig, Clone: 1H5C1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66184-1-Ig (P62,SQSTM1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

1X10^6 Jurkat cells were stained with 0.20ug P62,SQSTM1 antibody (66184-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH.

Non-treated HeLa cells and MG132 treated HeLa cells were subjected to SDS PAGE followed by western blot with 29503-1-AP (Phospho-P62,SQSTM1 (Ser349) antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

Immunofluorescent analysis of (-20°C Ethanol) fixed Chloroquine treated HepG2 cells using CL488-66184 (P62,SQSTM1 antibody) at dilution of 1:100. .

Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CL488-66184 (P62,SQSTM1 antibody) at dilution of 1:100.

Immunofluorescent analysis of (-20°C Ethanol) fixed Chloroquine treated U2OS cells using CoraLite®594 P62/SQSTM1 antibody (CL594-66184, Clone: 1H5C1 ) at dilution of 1:100, CL488-Phalloidin (green).

Immunofluorescent analysis of (-20°C Ethanol) fixed Chloroquine treated HepG2 cells using CoraLite®594-conjugated P62/SQSTM1 antibody (CL594-66184, Clone: 1H5C1 ) at dilution of 1:100.

1X10^6 HeLa cells were intracellularly stained with 0.2 ug CoraLite®595 Anti-Human P62,SQSTM1 (CL595-18420) (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

1X10^6 HeLa cells were intracellularly stained with 0.2 ug CoraLite®650 Anti-Human P62,SQSTM1 (CL650-18420) (red), or 0.2 ug APC-65128 ; APC Mouse IgG2b Isotype Control (APC-65128, Clone: MPC-11) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).