Various lysates were subjected to SDS PAGE followed by western blot with 18420-1-AP (P62/SQSTM1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
IP result of anti-P62/SQSTM1 (IP:18420-1-AP, 4ug; Detection:18420-1-AP 1:1000) with HEK-293 cells lysate 4000 ug.
WB result of P62/SQSTM1 antibody (18420-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-P62/SQSTM1 transfected HEK-293 cells.
Immunofluorescent analysis of (-20℃ Ethanol) fixed Starvation treated HepG2 cells using 18420-1-AP (P62/SQSTM1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunohistochemical analysis of paraffin-embedded human gliomas using 18420-1-AP (SQSTM1 antibody) at dilution of 1:50 (under 40x lens).
1X10^6 HEK-293T cells were stained with 0.2ug P62; SQSTM1 antibody (18420-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
