Various lysates were subjected to SDS PAGE followed by western blot with 18420-1-AP (P62/SQSTM1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
IP result of anti-P62/SQSTM1 (IP:18420-1-AP, 4ug; Detection:18420-1-AP 1:1000) with HEK-293 cells lysate 4000 ug.
WB result of P62/SQSTM1 antibody (18420-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-P62/SQSTM1 transfected HEK-293 cells.
Immunofluorescent analysis of (-20℃ Ethanol) fixed Starvation treated HepG2 cells using 18420-1-AP (P62/SQSTM1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunohistochemical analysis of paraffin-embedded human gliomas using 18420-1-AP (SQSTM1 antibody) at dilution of 1:50 (under 40x lens).
1X10^6 HEK-293T cells were stained with 0.2ug P62; SQSTM1 antibody (18420-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
WB result of P62/SQSTM1 antibody (66184-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-P62/SQSTM1 transfected HEK-293 cells.
L02 cells were subjected to SDS PAGE followed by western blot with 66184-1-Ig (P62/SQSTM1 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66184-1-Ig (P62/SQSTM1 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66184-1-Ig (P62/SQSTM1 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunofluorescent analysis of (-20℃ Ethanol ) fixed Starvation treated HepG2 cells using 66184-1-Ig(P62/SQSTM1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
1X10^6 Jurkat cells were stained with 0.20ug P62/SQSTM1 antibody (66184-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH.
Various lysates were subjected to SDS PAGE followed by western blot with 55274-1-AP (P62/SQSTM1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours
WB result of P62/SQSTM1 antibody (55274-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-P62/SQSTM1 transfected HEK-293 cells
Immunofluorescent analysis of ( -20℃ Ethanol ) fixed HepG2 cells using 55274-1-AP(P62/SQSTM1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Immunohistochemical analysis of paraffin-embedded human breast cancer using 55274-1-AP(SQSTM1 antibody) at dilution of 1:50 (under 40x lens)
Immunohistochemical analysis of paraffin-embedded human breast cancer using 55274-1-AP(SQSTM1 antibody) at dilution of 1:50 (under 10x lens)
