Daudi cells were subjected to SDS PAGE followed by western blot with 13179-1-AP (STAT5A/B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
K-562 cells were subjected to SDS PAGE followed by western blot with 13179-1-AP (STAT5A/B Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
3T3-L1 cells were subjected to SDS PAGE followed by western blot with 13179-1-AP (STAT5A/B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
DU 145 cells were subjected to SDS PAGE followed by western blot with 13179-1-AP (STAT5A/B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP result of anti-STAT5A/B (IP:13179-1-AP, 4ug; Detection:13179-1-AP 1:1000) with 3T3-L1 cells lysate 3200ug.
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13179-1-AP (STAT5A/B antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13179-1-AP (STAT5A antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
1X10^6 MCF-7 cells were stained with 0.2ug STAT5A/B antibody (13179-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.
Various lysates were subjected to SDS PAGE followed by western blot with 13179-1-AP (STAT5A antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours.
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 13179-1-AP (STAT5A/B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 13179-1-AP (STAT5A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).