Purity of recombinant human IL-2 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

recombinant human IL-2 (HZ-1015) stimulates dose-dependent proliferation of the CTLL-2 (mouse cytotoxic T) cell line. Viable cell number was quantitatively assessed by Prestoblue Cell Viability Reagent. CTLL-2 cells were starved for 5 hours before treatment with increasing concentrations of GMP recombinant human IL-2 for 48 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 values range from 0.05-0.35 ng/mL.

Purity of recombinant human IL-7 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

Recombinant human IL-7 (HZ-1281) stimulates dose-dependent proliferation of the 2E8 murine B Lymphocyte cell line. Cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. 2E8 cells were treated with increasing concentrations of recombinant IL-7 for 120 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 0.1-1.4 ng/mL.
 

Proteintech IL-7 (HZ-1281) demonstrates 3-fold lower EC50 and equivalent induction of proliferation compared to a leading competitor. Recombinant human IL-7 stimulates dose-dependent proliferation of the 2E8 murine B lymphocyte cell line. Cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. 2E8 cells were treated with increasing concentrations of recombinant IL-7 for 120 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 0.1-1.4 ng/mL.

Purity of recombinant human IL-2 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

GMP Recombinant human IL-2 (HZ-1015-GMP) induces dose-dependent proliferation of the CTLL2 (mouse cytotoxic T cell) cell line. Cell number was quantitatively assessed by PrestoBlue® cell viability reagent. CTLL2 cells were treated with increasing concentrations of GMP recombinant IL-2 for 48 hours. The EC50 was determined using a 4-parameter non-linear regression model. Activity determination was conducted in triplicate on a validated bioassay. The EC50 range is 0.05-0.35 ng/mL.

Purity of recombinant human IL-21 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

Recombinant human IL-21 (Cat no: HZ-1319) induces dose-dependent release of IFN-gamma in the NK-92 human cell line. NK-92 cells were treated with increasing concentration of recombinant IL-21 for 72 hours before supernatant collection. The supernatant was tested for IFN gamma using Proteintech’s AuthentiKine™ Human IFN-gamma ELISA Kit (KE00146). The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range in 0.25-1.25 ng/mL.

Purity of GMP-grade recombinant human IL-7 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

GMP recombinant human IL-7 (HZ-1281-GMP) stimulates dose-dependent proliferation of the 2E8 murine B lymphocyte cell line. Cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. 2E8 cells were treated with increasing concentrations of recombinant IL-7 for 120 hours. The EC50 was determined using a 4-parameter non-linear regression model. Activity determination was conducted in triplicate on a validated bioassay. The EC50 range is 0.1-1.4 ng/mL.
 

Purity of GMP-grade recombinant human IL-21 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

GMP Recombinant human IL-21 (Cat no: HZ-1319) induces dose-dependent release of IFN-gamma in the NK-92 human cell line. NK-92 cells were treated with increasing concentration of recombinant IL-21 for 72 hours before supernatant collection. The supernatant was tested for IFN gamma using Proteintech’s AuthentiKine™ Human IFN-gamma ELISA Kit (KE00146). The EC50 was determined using a 4-parameter non-linear regression model. Activity determination was conducted in triplicate on a validated bioassay. The EC50 range in 0.25-1.25 ng/mL.
 

Recombinant human IL-6 (Cat.NO. HZ-1019) stimulates proliferation of hybridoma (Proteintech mouse anti-GST clone 3G12B10) in a dose-dependent manner (blue curve, refer to bottom X-left Y axis). The activity of human IL-6 (1 ng/mL HZ-1019) is neutralized by mouse anti-human IL-6 monoclonal antibody 69001-1-Ig at serial dose (red curve, refer to top X-right Y axis). The ND50 is typically 10-30 ng/mL.

Recombinant human IL-6 (Cat.NO. HZ-1019) stimulates proliferation of hybridoma (Proteintech mouse anti-GST clone 3G12B10) in a dose-dependent manner (blue curve, refer to bottom X-left Y axis). The activity of human IL-6 (1 ng/mL HZ-1019) is neutralized by mouse anti-human IL-6 monoclonal antibody 69001-1-Ig at serial dose (red curve, refer to top X-right Y axis). The ND50 is typically 3-15 ng/mL. The NeutraControl mouse anti-human IL-6 monoclonal antibody 69501-1-Ig could recognize human IL-6 but could not neutralize human IL-6 activity in the range of 0.06 ng/mL-1000 ng/mL (green curve, refer to top X-right Y axis).

Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 69001-1-Ig (NeutraKine® IL-6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 69001-1-Ig (NeutraKine® IL-6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).