Various lysates were subjected to SDS PAGE followed by western blot with 22124-1-AP (TBC1D1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human testis slide using 22124-1-AP (TBC1D1 Antibody) at dilution of 1:50.

Immunohistochemical analysis of paraffin-embedded human testis slide using 22124-1-AP (TBC1D1 Antibody) at dilution of 1:50.

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 22124-1-AP (TBC1D1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 22124-1-AP (TBC1D1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

IP result of anti-TBC1D1 (IP:22124-1-AP, 4ug; Detection:22124-1-AP 1:1000) with Jurkat cells lysate 2400ug.

Non-treated HeLa and Calyculin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 29034-1-AP (Phospho-TBC1D1 (Ser660) antibody) at dilution of 1:10000 incubated at room temperature for 1 hours.

Non-treated and Calyculin A treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 81363-2-RR (Phospho-TBC1D1 (Ser660) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin antibody as loading control.

Non-treated and Calyculin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 81363-2-RR (Phospho-TBC1D1 (Ser660) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin antibody as loading control.

Non-treated and Calyculin A treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 81363-2-RR (Phospho-TBC1D1 (Ser660) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin antibody as loading control. This data was developed using the same antibody clone with 81363-2-PBS in a different storage buffer formulation.

Non-treated and Calyculin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 81363-2-RR (Phospho-TBC1D1 (Ser660) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin antibody as loading control. This data was developed using the same antibody clone with 81363-2-PBS in a different storage buffer formulation.

Non-treated and Calyculin A treated HEK-293T cells were subjected to SDS PAGE followed by western blot with 29068-1-AP (Phospho-TBC1D1 (Ser707) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Vinculin antibody as the loading control.