COLO 320 cells were subjected to SDS PAGE followed by western blot with 22006-1-AP (TBP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP Result of anti-TBP (IP:22006-1-AP, 4ug; Detection:22006-1-AP 1:500) with COLO 320 cells lysate 400ug.
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22006-1-AP (TBP Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).
1X10^6 HepG2 cells were stained with 0.20ug TBP antibody (66166-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH.
Western blot analysis of TBP in various cell lines using Proteintech antibody 66166-1-Ig at a dilution of 1:8000 incubated at room temperature for 1.5 hours.
IP Result of anti-TBP (IP:66166-1-Ig, 3ug; Detection:66166-1-Ig 1:800) with HEK-293 cells lysate 1800ug.
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66166-1-Ig (TBP Antibody) at dilution of 1:650 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunofluorescent analysis of (10% Formaldehyde ) fixed HeLa cells using 66166-1-Ig(TBP antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using CL488-66166 (TBP antibody) at dilution of 1:50.
1X10^6 HeLa cells were intracellularly stained with 0.2 ug CoraLite®488 Anti-Human TBP (CL488-66166, Clone:2H3B2) (red), or 0.2 ug Mouse IgG2a Isotype Control (CL488-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).