Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 13120-1-AP (TEAD3 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using 13120-1-AP (TEAD3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Various lysates were subjected to SDS PAGE followed by western blot with 13120-1-AP (TEAD3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.