HeLa cells were subjected to SDS PAGE followed by western blot with 11372-1-AP (TLE3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB result of TLE3 antibody (11372-1-AP, 1:500) with si-Control and si-TLE3 transfected HEK293 cells.
IP result of anti-TLE3 (IP:11372-1-AP, 4ug; Detection:11372-1-AP 1:2000) with HepG2 cells lysate 1080 ug.
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11372-1-AP (TLE3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TLE3 antibody (11372-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11372-1-AP (TLE3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).